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Reagents meet the rigorous standards established by AOAC and ASTM
Reagent kits are reproducible, lot-to-lot consistency, scalability, and easy to use
Evaluate 20 samples per hour, with as little as 10 - 50 μL sample volume
Each kit is configured and standardized for analysis of 110 samples at a cost of $4.5 per sample
Reagents are low hazard and require no toxic chemicals or solvents

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Total Glycerin Test Kits are designed to measure the amount of total, free, and bound glycerin in B100 biodiesel and biodiesel blends, an approach is consistent with the ASTM method D 6584. This test is designed for use by biodiesel manufacturers and to determine the glycerin content of finished biodiesel products.

  • Total Glycerin Overview
  • Total Glycercin Kits
  • Glycerin Analysis
  • Total Glycercin Protocols
Glycerin Analysis with Total Glycerin Kit
Why Total Glycerin?

Glycerol is an organic compound, also commonly called glycerin or glycerine. It is a colorless, odorless, viscous liquid that is a by-product of the biodiesel industry.

Glycerol is a by-product of the transesterification process. For every 1 ton of biodiesel that is manufactured, 100 kg of glycerol is produced. The presence of glycerin in biodiesel, whether free or in a combined form such as mono-, di-, or triacylglycerols, indicates its incomplete removal in the generation of the fuel. Residual glycerin does not burn well and can lead to injector coking, reduced storage, and filter plugging due to sediment formation.

Sample Preparation: Biodiesel blends and B 100 samples diluted 1:10 with preparation reagent.
Time Requirement: Sample Preparation < 5 minutes
Analysis time 10 minutes
Limit of Quantitation: 0.004% - 99.6% glycerin
Interferences: Sample containing glycerol and glycerol derivatives
Total Glycerin Kit

SafTest has the solution to rapid and repeatable testing for the biodiesel industry in the measurement of total, free, and bound glycerin.


To demonstrate method repeatability, accuracy, and linearity, over 30 samples were sent to the National Renewable Energy Laboratory (NREL) and 5 labs currently using the SafTest™ Biodiesel Platform. Samples were analyzed using ASTMCS Official method AOCS Aa 4-38 and the SafTest™ Percent Fat Kit.

Repeatability and Accuracy Studies

Saftest Total Glycerin
Total Glycerin Protocols

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