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RT-PCR (reverse transcription-polymerase chain reaction) is a common technique used for the detection and study of mRNA.

Two-step RT-PCR allows each part of the process to be individually optimized for higher yield and permits multiple PCRs from a single RT reaction.

Mastermix for first strand cDNA synthesis RT-&GO™ mastermix is a 2.5x concentrated mastermix for efficient first strand cDNA synthesis, containing all the components required for Reverse Transcription: M-MuLV Reverse Transcriptase, Reverse Transcription Buffer, RNase inhibitor and dNTPs. Random octamers may be used as universal primers in first strand cDNA synthesis, cDNA library construction and RT-PCR.

Description Pack Size Cat. No
Random Octamers 100 μl 11RTOCT100
RT-&GO™ Mastermix 100 Reactions 11RTRAG100

Achieve sensitive and consistent PCR results with ultra purified deoxynucleotides. Free of polymerase inhibitors each batch is specifically controlled for RNAse, DNAse and nicking contaminants activity.

This high quality standard is assured by stringent function testing in PCR on using genomic template. Suitable for use in PCR, RT-PCR, qPCR, cDNA synthesis, DNA sequencing and labeling.

Description Pack Size Cat. No
RNase Erase™ (to remove RNase contamination) 250 ml 04821682
DNA Erase™ (to remove DNA contamination) 500 ml 04821805
Water, DNA & RNAse Free 500 ml 04821932
dNTPs Set (100 mM each) 4 x 25 μmol 11NTACG100
dNTPs Mix (5 mM each) ready to use solution 5 μmol 11NTPMX050