ISIS DNA POL (1U/uL)

MP Biomedicals is an established manufacturer of PCR polymerases. Our customers are typically laboratories that demand the highest levels of reliability and who are not prepared to compromise on quality. The PCR product range covers requirements for general PCR, hot start, high fi delity, high sensitivity and multiplex PCR.

Ideal for high-fidelity amplification||Use to amplify DNA with difficult to read secondary structures||Optimal for blunt-end PCR cloning||The most thermostable polymerase available||Useful to amplify GC rich sequences

Isis™ DNA Polymerase is the most thermostable proofreading polymerase available for high fidelity PCR. Isis DNA Pol Kit contains Isis DNA polymerase, optimized incubation buffers with and without MgCl₂ and a separate MgCl₂.

Isis™ is a proofreading DNA polymerase and amplifi es DNA with 40 times more accuracy than standard Taq DNA polymerase. The error rate is 0.66 x 10⁻⁶ mutation frequency/bp/duplication as determined by the Flaman(1,2) method. Isis DNA polymerase is isolated from Pyrococcus abyssi(3,4) (Fig 1) and is an excellent choice for applications requiring high fi delity, like error-free cloning. Isis DNA Polymerase does not require primer 3’-end protection, unlike many other proofreading enzymes. This saves time during reaction setup and optimization. Isis DNA polymerase is able to amplify up to 6 kb on genomic DNA templates and 18 kb on phage DNA under a variety of amplification conditions (Fig 2). Isis DNA polymerase offers robust activity and flexibility.

One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74 °C under assay conditions.

Isis™