Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in LB Agar, Lennox formulations. Chosen reagents are combined in the proper proportions according to the formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Applications
LB Lennox Agar capsules is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. The ultimate in ease and convenience, LB Lennox Agar in a capsule form eliminates the waste, inaccuracies, and mess associated with weighing out bulk powder.The growth characteristics are the same as LB Lennox Agar powder. LB Lennox Agar Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
Product Overview
LB Lennox agar medium in capsules have same high quality formulation as LB Lennox agar powder with the added advantage of being in a capsule form, eliminating the need for weighing and handling. The growth characteristics are the same as LB Lennox agar powder.
Product Description
LB Lennox Agar medium is one of the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in an encapsulated format for convenience and ease of use. It is a rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.
Recommended Use
Add 35 capsules per liter of purified water and autoclave at 121 °C for 15 minutes.
Typical Working Concentration
35 g/L
Properties
Grade
Molecular Biology Certified™
Suitability
Suitable for use as a growth medium for Escherichia coli and Coliforms
Keywords
All Solid Media/Agars, Cloning, Culture Media, LB Broth and Agar, Media, Media by Microorganism, Microbial Media for Molecular Biology, Microbiology, Molecular Biology, Plant Biotechnology, Plant Molecular Biology, Prepared Escherichia Coli Media, Prepared Media, Products for UpStream Processes, Proteomics, Purification and Detection, Recombinant Protein Expression and Analysis
Key Applications
Culture growth media Cell culture media Proteomics Purification and Detection Recombinant Protein Expression and Analysis
Application Areas
Bacteria Cell Culture; Gene Expression; Microbiological Analysis; Transfection and Protein Expression
Product Type
Culture Media
Culture Media Type
Bacterial Media
Presentation
Capsules
Format
Capsules
Formulation
Typically contains 10 g Tryptone, 5 g Yeast Extract, 5 g NaCl, 15 g Agar. Small quantities of biological buffers have been added for proper pH adjustment.
pH
Acceptable pH range is 6.7 +/- 0.3 at 35 °C
Solubility
Soluble in water (35 g/L).
Storage & Handling
Store sterile LB Agar, Lennox at ambient temperature (15-30 °C). Powder medium is hygroscopic, therefore minimize exposure to air to prolong shelf life of media.
Certifications
Molecular Biology Certified™
Grade
Molecular Biology Certified™
Suitability
Suitable for use as a growth medium for Escherichia coli and Coliforms
Keywords
All Solid Media/Agars, Cloning, Culture Media, LB Broth and Agar, Media, Media by Microorganism, Microbial Media for Molecular Biology, Microbiology, Molecular Biology, Plant Biotechnology, Plant Molecular Biology, Prepared Escherichia Coli Media, Prepared Media, Products for UpStream Processes, Proteomics, Purification and Detection, Recombinant Protein Expression and Analysis
Key Applications
Culture growth media Cell culture media Proteomics Purification and Detection Recombinant Protein Expression and Analysis
Application Areas
Bacteria Cell Culture; Gene Expression; Microbiological Analysis; Transfection and Protein Expression
Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
RTECS Number
See MSDS
Protocols & References
References
François Delavat Deciphering the role of Paenibacillus strain Q8 in the organic matter recycling in the acid mine drainage of Carnoulès. Microbial Cell Factoties201211, NA
Etienne Coyaud Wide diversity of PAX5 alterations in B-ALL: a Groupe Francophone de
Cytoge´ne´tique He´matologique study. Blood2010115, 3089-3097