Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in LB Medium, Lennox formulations. Chosen reagents are combined in the accurate proportions according to the formula listed above and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid. The combinations were mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Applications
LB Medium Lennox capsules are a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. The growth characteristics are the same as L-Broth Agar powder. L-Broth supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component .
Product Overview
LB medium capsules have the same high quality formulation as LB powder with the added advantage of being in a capsule form, eliminating the need for weighing and handling. The growth characteristics are the same as LB powder.
Product Description
LB Medium, Lennox is a Molecular Biology Certified Bacterial Growth Medium. It is the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in a variety of formats for convenience and ease of use. It is Luria-Bertani, Rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.
Recommended Use
Add 20 capsules per liter of purified water and autoclave at 121°C for 15 minutes.
Properties
Suitability
Suitable for use as a growth medium for Microbial culture, Escherichia coli and Coliforms
Keywords
A - M, All Liquid Media/Broths, Broth / Media, Cloning, Core Bioreagents, Culture Media, Escherichia coli & Coliforms, HIS Select Supporting Products and Reagents, HIS-Select, LB Broth and Agar, Life Science Reagents for Cloning, Media, Media by Microorganism, Media from A - Z, Microbial Media for Molecular Biology, Microbiology, Molecular Biology, Nonselective, Plant Biotechnology, Plant Molecular Biology, Prepared Escherichia Coli Media, Prepared Media, Products for UpStream Processes, Proteomics, Purification and Detection, Recombinant Protein Expression and Analysis, Research Essentials
Key Applications
Culture growth media Cell culture media Proteomics Purification and Detection Recombinant Protein Expression and Analysis
Application Areas
Bacteria Cell Culture; Gene Expression; Transformation; Microbiological Analysis; Transfection and Protein Expression
Product Type
Culture Media
Culture Media Type
Bacterial Media
Kit Compatibility
All MP Plasmid Purification Kits including RapidPURE™, RPM™, and PhoenIX™
Presentation
Capsules
Formulation
Typically contains 10 g Tryptone, 5 g Yeast Extract, 5 g NaCl. Small quantities of biological buffers have been added for proper pH adjustment.
pH
Acceptable pH range is 6.9 ± 0.3 at 25 °C
Solubility
Soluble in water (20 g/L).
Sterility
Sterile
Storage & Handling
Store sterile LB Agar, Lennox at ambient temperature (15-30 °C). Powder medium is hygroscopic, therefore minimize exposure to air to prolong shelf life of media.
Certifications
Molecular Biology Certified™. Gelatin capsules are produced in a facility that meets the FDA’s recent guidance as being derived from BSE-free herds that are born, raised, and slaughtered in the United States.
Suitability
Suitable for use as a growth medium for Microbial culture, Escherichia coli and Coliforms
Keywords
A - M, All Liquid Media/Broths, Broth / Media, Cloning, Core Bioreagents, Culture Media, Escherichia coli & Coliforms, HIS Select Supporting Products and Reagents, HIS-Select, LB Broth and Agar, Life Science Reagents for Cloning, Media, Media by Microorganism, Media from A - Z, Microbial Media for Molecular Biology, Microbiology, Molecular Biology, Nonselective, Plant Biotechnology, Plant Molecular Biology, Prepared Escherichia Coli Media, Prepared Media, Products for UpStream Processes, Proteomics, Purification and Detection, Recombinant Protein Expression and Analysis, Research Essentials
Key Applications
Culture growth media Cell culture media Proteomics Purification and Detection Recombinant Protein Expression and Analysis
Application Areas
Bacteria Cell Culture; Gene Expression; Transformation; Microbiological Analysis; Transfection and Protein Expression
Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Safety
Avoid inhalation of media powder
WGK Germany
3
RTECS Number
See MSDS
Protocols & References
References
No references available for this product at this time.