Actin Monoclonal Antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. Also It's used in Indirect ELISA, Immunofluorescence, Immunoblots and Immunohistochemistry.
Product Description
The monoclonal actin (clone C4) is a mouse IgG1 (gamma 1 heavy/kappa light chain) antibody.
Typical Working Concentration
Indirect Immunofluorescence: 1:500 Immunoblots: 1:500 for DAB/peroxidase detection, actin band migrates at 42 kDa on SDS-PAGE. Immunohistochemistry: 1:500
Trade Name
ImmunO™
Properties
Keywords
Actin Antibodies, Antibodies, Antibodies for Cell Biology, Antibodies for Detecting Autophagy, Antibodies to Actin Isoforms, Antibodies to Cytoskeleton, Autophagy, Cell Biology, Cell Signaling and Neuroscience, Clone Index, Primary Antibodies
Application Areas
Immunoassays
Research Areas
Cell Structure
Product Type
Antibodies
Antibody Type
Primary Antibodies
Host
Mouse
Species Reactivity
Mammals, yeast, fungi
Presentation
Liquid
Format
Liquid
NOTES
This product is presented as filtered ascites containing 0.01% sodium azide as a preservative.
Storage & Handling
Store at -20°C. Storage at -70°C will give a longer storage time. Stable for weeks at 2-8°C. Avoid repeated freeze/thaw cycles.
Recommended Replacement For
869139
Packsize Numeric Quantity
100 µL||200 µL
Keywords
Actin Antibodies, Antibodies, Antibodies for Cell Biology, Antibodies for Detecting Autophagy, Antibodies to Actin Isoforms, Antibodies to Cytoskeleton, Autophagy, Cell Biology, Cell Signaling and Neuroscience, Clone Index, Primary Antibodies
Takeshi Ogihara Expression and function of Set7/9 in pancreatic islets. Landes Bioscience20091, 269-272
Kanako Ono Caenorhabditis elegans Kettin, a Large Immunoglobulin-like Repeat Protein, Binds to Filamentous Actin and Provides Mechanical Stability to the Contractile Apparatuses in Body Wall Muscle. Molecular Biology of cell200617, 2722–2734
Nathalie Velarde Diverse roles of actin in C. elegans early embryogenesis. BioMedCentral- Developmental Biology20077, NA
Isabel Rodriguez Escuredo Enteropathogenic Escherichia coli type III effectors alter cytoskeletal function and signalling in Saccharomyces cerevisiae. Microbiology2005151, 2933-2945
Deborah A. Barton Analysis of Cortical Arrays from Tradescantia virginiana at High Resolution Reveals Discrete Microtubule Subpopulations and Demonstrates That Confocal Images of Arrays Can Be Misleading. The Plant Cell200820, 982-994
Shenn X. Lu The Jumonji C Domain-Containing Protein JMJ30 Regulates Period Length in the Arabidopsis Circadian Clock. Plant Physiology2011155, 906-915
Eran Bosis Ssz1 Restores Endoplasmic Reticulum-Associated Protein Degradation in Cells Expressing Defective Cdc48–Ufd1–Npl4 Complex by Upregulating Cdc48. Genetics2010184, 695–706