GOAT ANTI -MOUSE IgG (H + L)

This product consists of 0.02M sodium phosphate, 0.15M NaCl, pH 7.6, 0.05% Sodium azide. It has been isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. It regulates several functions such as complement activation and phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-mouse IgG (whole molecule) antibody can be used in T cell proliferation assays. It can also be used in Ouchterlony Double Diffusion and immunoelectrophoresis. Goat anti- mouse IgG (whole molecule) antibody reacts specifically with mouse IgG subclasses IgG1, IgG2a, IgG2b and IgG3. The product has also shown no reactivity to human serum proteins.

IgG H&L CHAIN POLYCLONAL ANTIBODY can be used in Indirect immunofluorescence. It can also be used as a secondary antibody in western blotting to release NC1 domain of type IV collagen. Further, it can also be used in enzyme immunoassays and immunohistochemistry.

Anti-mouse IgG (whole molecule) is developed in goat using mouse IgG purified from pooled normal mouse serum as the immunogen.

ImmunO™