PEROXIDASE-CONJUGATED GOAT AFFINITY PURIFIED ANTIBODY TO MOUSE IMMUNOGLOBULINS (IgG, IgA, IgM)

To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with mouse gamma globulin. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is goat IgG; no trace of albumin is detected.

Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. No preservative is added.

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy) and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors.)

Product is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to mouse immunoglobulins (IgG, IgA, IgM).

Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.

Suggested working dilutions: 1:4,000 - 1:20,000 ELISA, 1:100 - 1:2,000 Immunoblotting, 1:50 - 1:200 Immunohistochemistry

Cappel™