PEROXIDASE-CONJUGATED GOAT AFFINITY PURIFIED ANTIBODY TO MOUSE IGG (WHOLE MOLECULE )

The total protein is measured using the Biuret procedure with bovine albumin as a standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with mouse IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is goat IgG; no trace of albumin is detected.

Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. No preservative is added.

IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid. These antibodies are capable of fighting bacterial and viral infection. They also regulate several functions like placental transport, cell surface-receptor binding and complement activation. Anti-mouse IgG (whole molecule)-peroxidase antibody can be used in ELISA. It can also be used in western blotting. Goat anti-mouse IgG-peroxidase antibody reacts specifically with mouse IgA, IgG1, IgG2a, IgG2b, IgG3 and IgM myeloma proteins.

Peroxidase-conjugated goat affinity purified antibody to mouse IgG is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors.) Anti-mouse IgG (whole molecule)-peroxidase antibody can be used as a secondary antibody in immunohistochemistry. It can also be used in Ouchterlony double diffusion and immunoelectrophoresis.

Product is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to mouse IgG (whole molecule) and buffer salts.

Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.

Cappel™