The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with mouse IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly goat IgG; no trace of albumin is detected.
Preparation Method
Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.
Applications
This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals).
Product Description
Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to mouse IgG (whole molecule) and buffer salts. Anti-Mouse IgG is developed in goat using purified mouse IgG as the immunogen. Affinity isolation removes essentially all goat serum proteins, including immunoglobulins which do not specifically bind to mouse IgG.
Recommended Use
Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted materia.
Trade Name
Cappel™
Properties
Concentration
Total Protein: 9.1 mg/mL; Titer: 1:199,000
Keywords
Secondary antibodies, anti-Mouse, IgG, Fluorophore, FITC, Immunology, Immunoglobulins, Heavy Chain IgG, Antibodies, Mouse Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies, Primary Antibodies, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies
Application Areas
Immunoassays
Product Type
Antibodies
Antibody Type
Secondary Antibodies
Antibody Category
Polyclonal
Conjugate
HRP
Host
Goat
Immunogen
Purified mouse IgG (whole molecule)
Species Reactivity
It shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG, some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Format
Lyophilized IgG
NOTES
shipped in: dry ice
Storage & Handling
Store lyophilized product at 2-8 °C. For long-term storage, aliquot and freeze reconstituted product at -20 °C or lower.
Packsize Numeric Quantity
2 mL
Concentration
Total Protein: 9.1 mg/mL; Titer: 1:199,000
Keywords
Secondary antibodies, anti-Mouse, IgG, Fluorophore, FITC, Immunology, Immunoglobulins, Heavy Chain IgG, Antibodies, Mouse Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies, Primary Antibodies, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies
Silvia Massa Mutants of plant genes
for developing cancer vaccines. Human Vaccines20117, 147-155
Brian N Chorley Differential Muc2 and Muc5ac secretion by stimulated guinea pig tracheal epithelial cells in vitro. Respiratory Research20067, NA
L. P. Daley-Bauer Contributions of conventional and heavy-chain IgG to immunity in fetal, neonatal, and adult alpacas.. Clinical and Vaccine Immunology20107, 2007–2015