The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to non-immunoglobulin serum proteins is not present.
Preparation Method
Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Background Information
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Anti-Mouse IgG (whole molecule)-FITC antibody reacts with normal mouse serum and all mouse IgGs.
Applications
Fluorescein-Conjugated Goat IgG Fraction to Mouse IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.
Product Description
Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse IgG (whole molecule) and buffer salts.
Recommended Use
Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Typical Working Concentration
Anti-Mouse IgG (whole molecule)-FITC antibody is suitable for use in immunohistochemistry (1:200) and immunofluorescence (1:100).
Trade Name
Cappel™
Properties
Concentration
Total Protein: 24.4 mg/mL; Antibody Protein: 10 mg/mL
Keywords
Antibodies, Antibody-Fluorochrome Conjugates, FITC Antibodies, Mouse Secondary Antibodies and Conjugates, Phosphorylation Analysis, Plant Biotechnology, Plant Immunochemistry, Post-Translational Modification, Primary Antibodies, Proteomics, Reagents for Antibody Detection, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies, Secondary Antibodies and Labeled Streptavidin
Key Applications
Immunofluorescence assays (IFA)
Application Areas
Immunoassays
Product Type
Antibodies
Antibody Type
Secondary Antibodies
Antibody Category
Polyclonal
Conjugate
FITC
Host
Goat
Immunogen
Mouse IgG
Species Reactivity
This product shows reactivity to mouse IgG; cross-reactivity to other species may exist.
Format
Lyophilized IgG
NOTES
F/P Ratio: 4.46 mol/mol
Storage & Handling
Store lyophilized product at 2-8 °C. Store reconstituted material no longer than 4 weeks at 2-8 °C. For long-term storage, aliquot and freeze reconstituted product at -20 °C or lower.
Packsize Numeric Quantity
2 mL
Concentration
Total Protein: 24.4 mg/mL; Antibody Protein: 10 mg/mL
Keywords
Antibodies, Antibody-Fluorochrome Conjugates, FITC Antibodies, Mouse Secondary Antibodies and Conjugates, Phosphorylation Analysis, Plant Biotechnology, Plant Immunochemistry, Post-Translational Modification, Primary Antibodies, Proteomics, Reagents for Antibody Detection, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies, Secondary Antibodies and Labeled Streptavidin
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strongly acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
Protocols & References
References
Joshua M. Thurman Altered renal tubular expression of the complement inhibitor Crry permits complement activation after ischemia/reperfusion. Journal of Clinical Investigation2006116, 357-368
Kerstin Radtke Plus- and Minus-End Directed Microtubule Motors Bind Simultaneously to Herpes Simplex Virus Capsids Using Different Inner Tegument Structures. Public Library Of Science Pathogens20106, NA