PEROXIDASE-CONJUGATED GOAT IGG FRACTION TO HUMAN IGG F(AB')₂

The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with human IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis.

Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.

Peroxidase-Conjugated Goat IgG Fraction to Human IgG F(ab')₂ is suitable for use as a reagent in enzyme immunoassays (EIA), cell and immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. Also used in Immunohistochemistry, EIA and Western blots.

Peroxidase-Conjugated Goat IgG Fraction to Human IgG F(ab')₂ is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG F(ab')₂ and buffer salts.

Immunohistochemistry: 1:500 - 1:5000; EIA and Western blots: 1:5000 - 1:100,000. Dilution recommended: 1:500 - 1:4,000 for Tissue Staining; 1:1,000 - 1:8,000 for Immuno Blotting; 1:2,000 - 1:16,000 for ELISA/FIA

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