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Blood is composed of several cell types that need to be routinely isolated. As of now, density gradient centrifugation appears to be the easiest, the fastest and the most reproducible method to isolate mononuclear cells from peripheral blood.

MP Biomedicals offers three distinct types of Ficoll-based fractionation solutions (LSM, LymphoSep and M-PRM)
to help you get the best of your blood samples!
Maximum yield of lymphocytes
Easy one-step centrifugation
Ready-to-use, sterile filtered formulation
Guaranteed >96% of isolated lymphocytes will be viable
Highly-cited in peer-reviewed scientific journals

This product consists in a sterile solution of sucrose polymer and diatrizoate salts which harbors a very specific density (1,077-1,080g/mL). Simple addition of LSM™ to the blood sample followed by a one-step centrifugation (400g / 30min), will allow perfect separation of RBCs (erythrocytes) and granulocytes from lymphocytes and other mononuclear cells (monocytes and platelets).

Available in several pack sizes, please click on specific product numbers to get more information.

Name Catalog Number Pack Size
LSM™ 0850494X 100 ml
0850494 5 x 100 ml
Bandara, G., Metcalfe, D. D., & Kirshenbaum, A. S. (2015). Growth of human mast cells from bone marrow and peripheral blood-derived CD34(+) pluripotent hematopoietic cells. Methods in Molecular Biology (Clifton, N.J.), 1220, 155–62. doi:10.1007/978-1-4939-1568-2_10
Iwata, S., Nakayamada, S., Fukuyo, S., Kubo, S., Yunoue, N., Wang, S.-P., … Tanaka, Y. (2015). Activation of syk in peripheral blood B cells in patients with rheumatoid arthritis: a potential target for abatacept therapy. Arthritis & Rheumatology (Hoboken, N.J.), 67(1), 63–73. doi:10.1002/art.38895
Otsuru, S., Hofmann, T. J., Raman, P., Olson, T. S., Guess, A. J., Dominici, M., & Horwitz, E. M. (2015). Genomic and functional comparison of mesenchymal stromal cells prepared using two isolation methods. Cytotherapy, 17(3), 262–270. doi:10.1016/j.jcyt.2014.10.013

By opposition to LSM™, LymphoSep™ is validated for In Vitro Diagnostic (IVD) usage and compatible with EDTA-treated blood samples thanks to its very specific composition of Ficoll and sodium metrizoate. It offers a quick, simple and reliable method for isolation of lymphocytes from whole blood. Similarly to LSM™, simply add in a 1:1 ratio LymphoSep™ solution to the blood sample, centrifuge 400g / 30min, and aspire the desired layer of lymphocytes.

Available in several pack sizes, please click on specific product numbers to get more information.
Name Catalog Number Pack Size
LymphoSep™ 091692249 100 ml
091692254 500 ml
Austin, E. D., Menon, S., Hemnes, A. R., Robinson, L. R., Talati, M., Fox, K. L., … West, J. (n.d.). Idiopathic and heritable PAH perturb common molecular pathways, correlated with increased MSX1 expression. Pulmonary Circulation, 1(3), 389–98. doi:10.4103/2045-8932.87308
Herrera, D., Rojas, O. L., Duarte-Rey, C., Mantilla, R. D., Angel, J., & Franco, M. A. (2014). Simultaneous assessment of rotavirus-specific memory B cells and serological memory after B cell depletion therapy with rituximab. PloS One, 9(5), e97087. doi:10.1371/journal.pone.0097087
Škorvaga, M., Nikitina, E., Kubeš, M., Košík, P., Gajdošechová, B., Leitnerová, M., … Belyaev, I. (2014). Incidence of common preleukemic gene fusions in umbilical cord blood in Slovak population. PloS One, 9(3), e91116. doi:10.1371/journal.pone.0091116

Mono-Poly Resolving Medium™ is a solution composed of a polysaccharide (Ficoll 400) and a radiopaque contrast medium (Hypaque) in a specific ratio to yield a density of 1,114±0,002. It is particularly useful for the separation of lymphocytes, monocytes and platelets (upper layer) from polymorphonuclear leukocytes (lower layer) and RBCs (pellet) in a one-step 300g/30min centrifugation.

Available in several pack sizes, please click on specific product numbers to get more information.
Name Catalog Number Pack Size
M-PRM™ 091698049 100 ml
Francischetti, I. M. B., Ma, D., Andersen, J. F., & Ribeiro, J. M. C. (2014). Evidence for a lectin specific for sulfated glycans in the salivary gland of the malaria vector, Anopheles gambiae. PloS One, 9(9), e107295. doi:10.1371/journal.pone.0107295
Chagas, A. C., Oliveira, F., Debrabant, A., Valenzuela, J. G., Ribeiro, J. M. C., & Calvo, E. (2014). Lundep, a sand fly salivary endonuclease increases Leishmania parasite survival in neutrophils and inhibits XIIa contact activation in human plasma. PLoS Pathogens, 10(2), e1003923. doi:10.1371/journal.ppat.1003923
Su, H.-W., Prieto, J. L., & Voldman, J. (2013). Rapid dielectrophoretic characterization of single cells using the dielectrophoretic spring. Lab on a Chip, 13(20), 4109–17. doi:10.1039/c3lc50392e