RNA Isolation & Purification Kits

The FastRNA™ SPIN Kit for Microbes quickly and efficiently isolates high-quality, total RNA from tough-to-lyse bacterial cell cultures and other microbial sources in approximately 15 minutes using a zirconium silicate Lysing Matrix (0.1mm) to lyse bacteria and SPIN columns for the purification process.

>> FastRNA™ Spin Kit for Microbes Pricing and Information

The FastRNA™ SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast strains, fungi, and algae in approximately 15 minutes using a specialized Lysing Matrix (Yttria-stabilized zirconium oxide, 0.5mm) for cell lysis and SPIN columns for the purification process.

>> FastRNA™ Spin Kit for Yeast Pricing and Information

FastRNA™ Pro Soil Kits

Isolate Total RNA from Soil!

• Easily lyse difficult gram positive cells, plant material, and organic debris directly from soil
• Lysis and purification solutions protect RNA during processing
• Humic acids levels reduced to allow uninhibited RT-PCR

The FastRNA™ Pro Soil-Direct and Indirect kits are designed to efficiently isolate total RNA from organic material found in soil samples or soil supernatants. The direct method consists of extracting nucleic acid from microorganisms and other biological specimens directly from soil. The indirect method utilizes an initial separation of microorganisms and other biological specimens from the soil followed by lysis of the organisms and RNA purification. This method also permits soil incubation with growth media in order to amplify under-represented living organisms prior to RNA isolation if specific comparisons of microbial diversity are not desired. FastRNA™ Pro Soil kits purify RNA in a process that removes humic substances and other inhibitors, and efficiently inactivates cellular RNases during homogenization to prevent RNA degradation. Purified RNA is thus suitable for RT-PCR analysis and other downstream applications.

Kit Components: RNAPro™ Soil Lysis Solution, Lysing Matrix E, Phenol:Chloroform (1:1) (Direct Kit), Inhibitor Removal Solution, DEPC-treated H2O, RNAMATRIX™ Binding Solution, RNAMATRIX™ Slurry, RNAMATRIX™ Wash Solution Concentrate, Quick-Clean Spin Filters, Filtration Cloth, (Indirect Kit)

The FastRNA™ Pro Soil-Direct Kit is designed to efficiently isolate total RNA from organic material found in soil samples. Using any FastPrep instrument, up to 0.5g of material is homogenized by Lysing Matrix E impact-resistant 2ml tubes. Total RNA is released into a protective solution, extracted, precipitated and cleaned with the proprietary RNAMATRIX and a Quick-Clean Spin Filter. Total RNA prepared with this kit is suitable for RT-PCR and other downstream applications.

>> FastRNA™ Kit for Soil Direct Pricing and Information

The FastRNA™ Pro Soil-Indirect Kit is designed to efficiently isolate total RNA from organic material found in soil supernatants. Cellular material is washed away from soil particles, centrifuged, and homogenized by any FastPrep® Instrument in impact-resistant 2 mL tubes of Lysing Matrix E. Total RNA is released into a protective solution, extracted, precipitated, and cleaned with the proprietary RNAMATRIX™ and a Quick-Clean Spin Filter. Total RNA prepared with this kit is suitable for RT-PCR analysis and other downstream applications.

>> FastRNA™ Kit for Soil Indirect Pricing and Information

FastRNA™ Pro Kits

Isolate High Quality Total RNA with a Single-Reagent Extraction Method!

• Safe and consistent RNA isolation with the single-reagent RNAProTM solution
• Lyse and isolate total RNA from a variety of sample types

The FastRNA™ Pro Kits are designed to quickly and efficiently isolate total RNA from virtually any sample. During the homogenization step, intact total RNA is released in the proprietary RNAPro™ solution where it is immediately stabilized. The RNAPro™ solution inactivates cellular RNases during cell lysis to prevent RNA degradation. RNA is then extracted with chloroform and precipitated with ethanol. DEPC-treated water is provided for re-suspension of total RNA. High quality RNA prepared with FastRNA™ Pro Kits is ready for all downstream applications including RT-PCR, gene expression and microarray analysis.

Kit Components: Lysing Matrix B (Blue Kit), Lysing Matrix C (Red Kit), Lysing Matrix D (Green Kit), RNAproTM Solution, DEPC-treated H2O

The FastRNA® Pro Blue Kit is designed to efficiently isolate total RNA from gram positive and gram negative bacteria. Using any FastPrep Instrument, up to 1010 cells are homogenized by Lysing Matrix B in impact-resistant 2 mL tubes. Total RNA is released into the proprietary, protective RNApro™ Solution. Following extraction with chloroform and precipitation, total RNA is extracted.

>> FastRNA™ Pro Blue Kit for Bacteria Pricing and Information

The FastRNA™ Pro Red Kit is designed to efficiently isolate total RNA from yeast and fungi. Using any FastPrep® Instrument, up to 109 cells are homogenized by Lysing Matrix C in impact-resistant 2 mL tubes. Total RNA is released into the proprietary, protective RNApro™Solution. Following extraction with chloroform and precipitation, total RNA is extracted.

>> FastRNA™ Pro Red Kit for Soil Pricing and Information

The FastRNA™ Pro Green Kit is designed to efficiently isolate total RNA from any type of plant and animal tissue or cultured cells. Using any FastPrep® Instrument, between 50-500 mg of tissue can be homogenized by Lysing Matrix D in impact-resistant 2 mL tubes. Total RNA is released into the proprietary, protective RNApro™ Solution. Following extraction with chloroform and precipitation, total RNA is extracted.

>> FastRNA™ Pro Green Kit for Plants and Animals Pricing and Information