The FastPROTEIN™ products employ a powerful, patented technology for the rapid lysis of yeast and bacteria. Used in conjunction with any FastPrep® Instrument, these products offer the fastest way to release expressed proteins from the host organism. FastPROTEIN™ Kits are perfect for analyzing protein expression conditions using gel analysis. Samples are enclosed during the quick lysis step, thus preventing cross-contamination or sample loss. Save valuable time by reducing sample lysis time to seconds! Total proteins isolated with the FastProtein™ Matrices are native and can be used for a variety of applications including SDS-PAGE, Western Blotting, immunoprecipitation, gel mobility shift assays, and enzyme activity analysis.
Protein Isolation Kits for Any FastPrep™ Homogenizer
The FastPROTEIN™ Blue matrix is optimal for lysing gram positive and gram negative bacteria. These fine glass beads are designed for use with gram positive bacteria or any difficult microorganism. Cells, resuspended in either 1X PBS or your own expression buffer, are added to the Lysing Matrix and processed in any FastPrep® Instrument for 20-40 seconds.
The FastPROTEIN™ Red matrix is used to lyse yeast cells. Cells, re-suspended in either Yeast Breaking Buffer (YBB-supplied with the kit) or your own expression buffer, are added to the small glass beads of this Lysing Matrix and processed in any FastPrep® Instrument for 20-40 seconds.
An optimized Lysing Matrix A, coupled with any FastPrep® Instrument, quickly lyses most tissue samples in 40 seconds or less. Following lysis, samples are loaded to the ConA lectin resin SPIN filter tubes. The resin is washed and the Glycoproteins are eluted with the elution buffer. Eluted Glycoproteins are ready for 1-D gel electrophoresis and total protein (Bradford type) Assays. Other downstream applications may require sample processing to remove incompatible substances in the elution buffer.
An optimized Lysing Matrix A, coupled with any FastPrep® Instrument, quickly lyses most tissue samples in 40 seconds or less. Following lysis, samples are loaded to the WGA lectin resin SPIN filter tubes. The resin is washed and the Glycoproteins are eluted with the elution buffer. Eluted Glycoproteins are ready for 1-D gel electrophoresis and total protein (Bradford type) Assays. Other downstream applications may require sample processing to remove incompatible substances in the elution buffer.